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E-mail: jhpark@ynu.ac.krReceived January 28, 2013, Accepted March 24, 2013Sulfated β-cyclodextrin (SCD)-coated zirconia monolith was used as the chiral stationary phase in capillaryelectrochromatography for enantiomeric separation of basic chiral compounds. SCD adsorbed on the zirconiasurface provided a stable chiral stationary phase in reversed-phase eluents. Retention, chiral selectivity andresolution of a set of six basic chiral compounds were measured in eluents of varying pH, composition ofmethanol and buffer. Optimum mobile phase condition for the separation of the compounds was found to bemethanol content of 30%, buffer concentration of 30 mM and pH of 4.0.Key Words : Chiral separation, Capillary electrochromatography, Zirconia monolith, Sulfated β-cyclodextrinIntroductionCapillary electrochromatography (CEC) on monolithiccolumns are becoming attractive alternative to particle-packed columns in HPLC and electrochromatography.

Chiral Separation of Basic Compounds on Sulfated β-Cyclodextrin-Coated Zirconia Monolith by Capillary Electrochromatography