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We nowwish to report that α-chymotrypsin (α-CT) was also activatedby added IONs in organic solvent and IONs-activated α-CTcatalyzed the synthesis of peptides efficiently.In typical procedures, α-CT from its commercial sourceswas lyophilized in the presence of 1% phosphate buffer (pH7.8). The lyophilized enzyme powder and IONs (averagesize 12 nm)

Activity of α-Chymotrypsin Enhanced in the Presence of Iron Oxide Nanoparticles in Organic Solvent: Application to Peptide Synthesis