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Department of Chemistry, Quaid-i-Azam University, Islamabad-45320, PakistanReceived April 4, 2012, Accepted May 29, 2012A new series of 2,5-disubstituted-1,3,4-thiadiazoles 6a-i was synthesized by overnight stirring various 1,4-disubstituted thiosemicarbazides 5a-i in polyphosphoric acid followed by neutralization. The structures ofnewly synthesized compounds 5a-i and 6a-i were characterized by IR,

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Synthesis, Urease and Acetylcholine Esterase Inhibition Activities of Some 1,4-Disubstituted Thiosemicarbazides and their 2,5-Disubstituted Thiadiazoles