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In pH 4.2 HAc-NaAc buffer solution, horseradish peroxidase (HRP) catalyzed H2O2 oxidation of nanosilver to form Ag+. After centrifugation, Ag+ in the supernatant can be measured by graphite furnace atomic absorption spectrophotometry (GFAAS) at the silver absorption wavelength of 328.1 nm. When HRP concentration increased, the Ag+ concentration in the supernatant increased, and the absorption value enhanced. The HRP concentration in the range of 0.84-50 ng ·mL-1 was linear to the enhanced absorption value (ΔA), with a regression equation of ΔA=0.012C+0.11, correlation coefficient of 0.9988, and detection limit of 0.41 ng·mL-1 HRP. The proposed GFAAS method was used to detect HRP in waste water samples, with satisfactory results.

Graphite Furnace Atomic Absorption Spectrophotometric Determination of Trace Horseradish Peroxidase Using Nanosilver