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E-mail: kimde@konkuk.ac.krReceived August 6, 2011, Accepted August 16, 2011Key Words : DNA helicase, RNA synthesis, Run-off transcript, T7 RNA polymeraseIn vitro transcription using the bacteriophage RNA poly-merase is an efficient way to synthesize RNA molecules in alarge scale. There are several RNA polymerases and eachenzyme needs a different DNA promoter sequence forinitiation of transcription.

DNA Helicase Reduces Production of Aberrant Run-off Transcripts during in vitro RNA Synthesis with T7 RNA Polymerase