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Figure 1. Overexpression of DUSP26 in macrophages inhibits LPSstimulated TNF-α production. RAW264.7 cells were transfected with either the empty vector or the construct expressing FLAG-DUSP26. After 16 h stimulation with LPS (1 μg/mL), supernatants were analyzed for TNF-α production using an ELISA assay as described in the Experimental Section. Cell lysates were subjected to immunoblotting using an anti-FLAG antibody for detection of DUSP26. The results presented are representative of three independent experiments. Error bars indicate ±SEM.

Dual-Specificity Protein Phosphatase 26 (DUSP26) Inhibits LPS-Induced TNF-α Production