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Figure 1. Inhibition of LPS-stimulated TNF-α production by PTPH1 in macrophages. RAW264.7 cells were transfected with either the empty vector or the construct bearing FLAG-PTPH1. After 16 h stimulation with LPS, supernatants were analyzed for TNF-α production using an ELISA assay, as described in the Experimental Section. Cell lysates were subjected to immunoblotting using an anti-FLAG antibody for detection of PTPH1. The results presented are representative of three independent experiments. Error bars indicate ± SEM.

Protein Tyrosine Phosphatase H1 (PTPH1) Inhibits LPS-induced TNF-α Production