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The thermal stability and faithful replication of a natural DNA duplex is based on the Watson-Crick hydrogen-bonding (H-bonding) patterns of dA:dT and dC:dG base pairs. Additional stable and selective unnatural base pairs would facilitate hy-bridization and encoding experiments in cases where natural sequences cross hybridize or where increased information storage is desirable.

2-Aminobenzene Derivatives as Unnatural Nucleobases and Their DNA Duplex Stabilities