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A brush type chiral stationary phase (CSP 2) derived from α-amino β-lactam was prepared for the separation of the enan- tiomers of β-blockers. Compared to the CSP derived from α-amino phosphonate (CSP 1), in general, the conforma- tionally rigid CSP 2 showed greater scope and much enhanced enantioselectivity for the resolution of β-blockers. The effect of various salt additives on enantioseparation of β-blockers in the mobile phase was investigated. The unusual effect of temperature on the chromatographic behaviors was observed on CSP 2. It also afforded appreciable increases in enantioselectivity without significantly affecting resolution, as the column temperature was reduced.

Separation of the Enantiomers of β-Blockers Using Brush Type Chiral Stationary Phase Derived from Conformationally Rigid α-Amino β-Lactam