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-methylation on DNA. The struc-tural and/or dynamic studies of the GATC site with various 6methylation modifications are required in order to elucidate the mechanism of specific recognition SeqA to hemimethylated GATC in a sea of fully methyl ated one. Recently, the kinetics and thermodynamics of base-pair openings of the unmethylated (UMe), hemimethylated (HMe), and fully methylated (FMe) GATC-containing DNA duplexes using tris(hydroxymethyl) aminomethane (TRIS) were reported.

Base Pair Opening Dynamics in Methylated GATC Sites Catalyzed by Ammonia