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Department of Animal Science, Chonnam National University, Gwangju 500-757, Korea Received July 25, 2008Biofilm contains heterogeneous three-dimensional structures composed of extracellular polymeric substance(EPS), which are greatly influenced by flow conditions. Here, we report a microfluidic platform highly suitablefor nanoscale investigation of biofilms formed under laminar flows. This is possible because biofilms formedon glass beads having an average diameter of about 200 µm in the microfluidic device can be easily taken outand located for imaging under high resolution microscopes, such as atomic force microscope (AFM) andscanning electron microscope (SEM). Escherichia coli formed biofilms in the device at various flow conditions(0-50 µL min

bulletin of the korean chemical society

Nanoscale Characterization of Escherichia coli Biofilm Formed under Laminar Flow Using Atomic Force Microscopy (AFM) and Scanning Electron Microscopy (SEM)