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Autokinase, ATP, FRET, TMRThe Kin.46 kinase ribozyme was selected for the transferof the thiophosphate from ATP γS to its own 5' hydroxyl end.The reaction requires an oligonucleotide effector that iscomplementary to the 3' primer binding sequence (PBS) ofthe ribozyme and this effector served as reverse transcriptionprimer during the amplification steps of the original selec-tion for activity (Fig. 1).

Proximity of Both Ends of Stems P3 and P4 of Self-kinasing RNA by ATP