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Determination of Co(II) ion as a 4-(2-thiazolylazo)re sorcinol(TAR) or 5-methyl-4-(2-thiazolylazo)resorcinol (5MTAR) chelate was accomplished by reversed-phase capillary high-performance liquid chromatography (RP-Capillary-HPLC) using a Vydac C4 column and MeCN-water mixture as mobile phase. The effect of change in pH and MeCN percentage of the mobile phase on the retention factor, k and peak intensity were evaluated. It was found that 30% MeCN (v/v) of pH 5.60 or 7.20 was adequate as mobile phase when TAR or 5MTAR is used. Detection limit (D.L., S/N=3) in each case was 2.0 × 10 −7 M (11.8 ppb) and 3.0 × 10 −7 M (17.7 ppb). The Co(II) ion in mineral and waste water was determined with the optimum column and mobile phase.

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Determination of Co(II) Ion as a 4-(2-Thiazolylazo)resorcinol or 5-Methyl-4-(2-thiazolylazo)resorcinol Chelate by Reversed-Phase Capillary High-Performance Liquid Chromatography