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The microphase adsorption - spectral correction (MPASC) technique was described and applied to study the interaction of fluorescein isothiocyanate (FITC) with cetyl trimethylammonium bromide (CTAB). The synergism mechanism of micelle was analyzed and discussed. The aggregation of FITC on CTAB obeys the Langmuir monolayer adsorption. Results showed that the monomer and micellar aggregate is FITC-CTAB 3 and (FITC-CTAB 3 ) 2 9 , respectively at pH 9.62. The adsorption constant of the CTAB-FITC aggregate was determined to be 2.10 x 10 5 . Interestingly, it was observed for the addition of an anionic surfactant to desorb FITC from its CTAB aggregate and this has been applied to the quantitative determination of trace amounts of anionic detergent (AD) in sewage.

bulletin of the korean chemical society

Langmuir Aggregation of Fluorescein Isothiocyanate (FITC) on Cetyl Trimethylammonium Bromide (CTAB) and Application to Determination of Anionic Detergent in Sewage