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Recently, a number of successful research reports are accumulated to increase the carotenoid level in potato tuber such as β-carotene, precursor of vitamin A and keto-carotenoid like astaxanthin in which is not synthesized in most plants tissue since it does not contain a specific enzyme to add keto-ring in carotenoid molecule. In particular, keto-carotenoids are more interested due to their strong antioxidant activity. Currently, the content of β-carotene was increased up to 3,600-fold (47 ㎍/g dry weight) when compared to the control potato tuber, parental cultivar for genetic modification. In addition, astaxanthin, one of the major keto-carotenoid was accumulated up to 14 ㎍/g dry weight in potato tuber with red color by over expressing the gene encoding β- carotene ketolase isolated from marine microorganisms. In this article, we summarized carotenogenesis-related genes that have been used for metabolic engineering of carotenoid biosynthetic pathway in potato. Furthermore, strategies for the accumulation of carotenoids and keto- carotenoids in specific potato tuber, bottle necks, and future works are discussed.

Development of transgenic potato with high content of functional carotenoids by using metabolic engineering