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The aim of this study is to evaluate the effects of herbal alternative (green tea polyphenols) and ethylenediaminetetraacetic acid (EDTA) + NaOCl on the microhardness of root canal dentin after instrumentation with ProTaper Next (PTN; Dentsply) and Twisted Files (TF; SybronEndo). Materials and methods: Twenty single-rooted human premolar teeth will be instrumented up to the working length with PTN and TF (10 each) and then will be longitudinally sectioned into 40 segments and then embedded in an autopolymerizing acrylic resin. The microhardness of the dentin in the specimen will be measured with a Vickers diamond indenter at the apical third of the roots. Then the specimens will be divided randomly into two groups: 17% EDTA + 2.5% NaOCl and green tea polyphenols. Posttreatment microhardness values will be obtained and the decrease in microhardness will be calculated as a percentage. Microhardness values will be statistically analyzed using the Kruskal–Wallis and Mann–Whitney U tests. Results: Statistical analysis using one-way analysis of variance (ANOVA) showed that both the file groups with NaOCl + EDTA showed significant reduction in root dentin microhardness (p > 0.05). Conclusion: Even though green tea polyphenols showed lesser reduction in microhardness, its ability to remove smear layer completely and the antibacterial efficacy and practicality of using green tea as a root canal irrigant require further investigations. Its potency to discolor tooth and ability to eliminate biofilm is a matter of debate.

conservative dentistry and endodontic journal

Effect of Herbal Alternative Irrigant (Green Tea Polyphenols) on the Microhardness of Root Canal Dentin after Instrumentation with Thermomechanically Treated NiTi Instruments: An in vitro Study