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Context: Infection rates in the intensive care unit (ICU) have been found to be the highest among all hospital-acquired infections. Aim: To determine the prevalence of current bacteriological profile in blood cultures along with their antibiogram from ICU patients. Settings and design: The present study was conducted in the Department of Microbiology, over a period of 1 year, i.e., from September 2016 to August 2017. Materials and methods: A total of 2,028 blood cultures were received from various ICUs. The isolates were identified by standard microbiological procedures, and antibiogram was determined by Clinical and Laboratory Standards Institute (CLSI) guidelines. Statistical analysis: Qualitative data were presented as mean and standard deviation and quantitative data as proportions. Association was tested using chi-squared test. Statistical significance was considered when p < 0.05. Statistical Package for the Social Sciences version 20.0 software was used for analysis. Results: Total positive cultures were obtained in 504 (24.86%) cases. Among the Gram-positive (GP) isolates 288 (57.14%), coagulase-negative Staphylococci (CoNS) 55.5% was the most common followed by Staphylococcus aureus 34% and Enterococcus spp. 10.4%. Acinetobacter baumannii 52.3% was the most common Gram-negative (GN) isolate, 216 (42.85%), followed by Escherichia coli 27.7%, Klebsiella pneumoniae 14.35%, and Pseudomonas aeruginosa 5.5%. Most of the GP isolates were susceptible to vancomycin and linezolid and GN isolates to imipenem and meropenem. Conclusion: This study shows the high number of positive blood cultures in ICUs, which can be attributed to increasing use of intravenous devices, interventions, and immunosuppression. Hence, this study may be a useful guide for initiating early empiric therapy for ICU patients and will help in formulation of antibiotic policy in our institute.

Bacteriological Profile and Antibiogram of Aerobic Blood Culture Isolates from Intensive Care Units in a Teaching Tertiary Care Hospital