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Aims: Our study focused on the fabrication of platelet-rich fibrin (PRF) and evaluated its influences on cell behaviors, including proliferation and migration. Materials and methods: Platelet-rich fibrin was prepared from human peripheral blood according to Choukroun’s method without using nonanticoagulant and foreign factors for platelet activation. Platelet-rich fibrin architecture was studied by hematoxylin and eosin staining. The investigation of PRF effects on human gingival fibroblasts (hGFs) was conducted via PRF liquid extract. Cell proliferation was determined via the number of cells after a period of time incubated in PRF liquid extract. Influence of PRF liquid extract on the migration of hGFs was conducted via scratch wound healing assay. Results: Histological staining reviewed the natural fibrin fiber matrix of PRF. Platelet-rich fibrin liquid extract promoted hGF proliferation after 7 days of cultivation. Human gingival fibroblast proliferation in PRF liquid extract was more superior than those cultured in complete medium. Platelet-rich fibrin was also found to be able to promote the migration of hGFs for up to 48 hours. Conclusion: These results indicated that PRF is suitable to be used as autologous natural biomaterial in supporting wound healing and in further application in periodontitis treatments.

Platelet-rich Fibrin Influences on Proliferation and Migration of Human Gingival Fibroblasts