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The aim of this study was to verify the influence of different energy densities of low-level laser therapy (LLLT) on the viability and proliferation of pulp fibroblasts of human primary teeth (HPF). Cultures of fibroblast were irradiated with LLLT according to experimental groups: I (1.2 J/cm2), II (2.5 J/cm2), III (3.7 J/cm2), IV (5.0 J/cm2), V (6.2 J/cm2), and VI (not irradiated). Cell viability was determined 6 and 24 hours after of irradiation using 3-(4, 5-dimethyl-2-thiazolyl) -2, 5-diphenyl -2Htetrazolium bromide (MTT) assay. Cell proliferation was assessed through sulforhodamine B (SRB) assay after 24, 48, and 72 hours of irradiation. Data were submitted to two-way analysis of variance (ANOVA) and Tukey test (p < 0.05). After 6 hours of irradiation, group III exhibited the greatest cell viability compared with other groups. After 24 hours, group I exhibited the smallest absorbance value compared with group VI. To cell proliferation, 24 and 48 hours after irradiation, all groups showed similar results to those of group VI. After 72 hours, group I showed lower cell proliferation than the others. Group V exhibited results very similar to those of group VI. This study suggests that different energy densities of LLLT influenced the viability and proliferation of HPF.

Low-level Laser Therapy Effects on Pulp Cells from Human Primary Teeth