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Ab s t r Ac t Aim: To evaluate the effect of surface microtopography on osteogenic cell behavior. Materials and methods: Commercially pure grade II titanium discs of similar design and dimensions (2.5 mm × 6 mm) were selected for the study. Samples were sandblasted using 110 μm grit-size alumina (Al2O3), and 15% hydrofluoric acid (HF), 96% sulphuric acid (H2SO4), and 37% hydrochloric acid (HCl) were used for acid etching. Scanning electron microscopy (SEM) with energy-dispersive X-ray spectroscopy (EDX) of samples was performed to observe the surface morphology and elemental analysis. Surface roughness was measured using a surface profilometer. Human osteosarcoma (HOS) cells were seeded at a density of 5 × 103 per test material and control cover slip and incubated for 48 hours. After critical point drying and gold sputtering, a scanning electron microscope was used to observe the cell morphology, proliferation, and cell attachment. Results: Scanning electron microscopy revealed that sandblasting and acid etching resulted in a homogeneous rough surface with a flatter profile. Energy-dispersive X-ray spectroscopy showed a significant increase in the oxygen content (29.38%) after sandblasting and acid etching. Conclusion: Scanning electron microscopy result of the sandblasted and acid-etched surface showed that cell sheets were able to migrate into the pores and adhered inside the valleys suggesting excellent sign of osseointegration.

international journal of prosthodontics and restorative dentistry

Influence of Sandblasted and Acid-etched Commercially Pure Grade II Titanium Surface on Human Osteosarcoma Osteoblast Cell Proliferation and Attachment: A Pilot Study