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Materials and methods: Fifty extracted human single rooted teeth were selected and each test group had 25 teeth. The teeth were decoronated and then prepared with protaper rotary files till the no F2 in conjunction with 17% EDTA lubrication and 2 ml of 5.25% sodium hypochlorite irrigation. The teeth in group I were obturated using the zinc oxide sealer mixed with triple antibiotic paste and the teeth in group II were obturated using the zinc oxide sealer alone and then nail varnish was applied leaving the apical 3 mm of root exposed. The apical third was then immersed in 5 ml of 2% methylene blue dye in 15 ml screw capped bottle for 72 hours. The varnish was removed and the teeth were subsequently immersed in 35% nitric acid and kept again for 72 hours in a centrifugal tube. After the teeth were completely dissolved the solution was then filtered with a fine grit filter paper and centrifuged at 2000 rpm for 1 minute. The collected solution was then subjected to spectrophotometric analysis. The data was evaluated statistically by Mann-Whitney U-test.

international journal of prosthodontics and restorative dentistry

To Compare and Evaluate the Sealing Ability of Root Canal Sealer with and without Triple Antibiotic Paste using Ultraviolet-visible Spectrophotometric Analysis