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Ab s t r Ac t Aim: This preliminary study aimed to evaluate the frequency of human papillomavirus (HPV) and Epstein-Barr virus (EBV) DNA in solid/multicystic ameloblastoma (AM). Materials and methods: A total of 20 fresh-frozen AM tissue was investigated for HPV and EBV DNA using polymerase chain reaction (PCR) at L1 of HPV and LMP1 of EBV. Results: It was found that 2 out of 20 cases (10%) were positive for HPV PCR, whereas 3 out of 20 cases (15%) were positive for EBV PCR. Importantly, a mix of HPV and EBV DNA was observed in one case. Conclusion: Our study presented a low frequency of HPV and EBV DNA in AM. Further studies with different cohorts and larger sample sizes are necessary for a better understanding of the frequency of both viruses in AM. Clinical significance: HPV and EBV may not be involved in ameloblastogenesis.

Low Frequency of Human Papillomavirus and Epstein-Barr Virus DNA in Ameloblastoma of Thai Patients