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Hydrogen peroxide (H2O2) is an effective bleaching agent of tooth whitening, but its use causes changes in the chemical composition of the elements that configure tooth enamel. The purpose of this study is to determine whether there are changes in the composition of the elements that configure the tooth enamel after bleaching using 35% H2O2. Materials and methods: This study was performed in vitro, which is an experimental research laboratory with controlled time series design. The samples used in this study were six permanent incisors postextraction and are divided into treatment and control groups. The control group was soaked in saline and treatment groups applied with H2O2 35%, with time series of 1, 1.5, 2, 2.5, and 3 hours. The elemental composition of enamel was observed using energy-dispersive X-ray spectroscopy analysis; results can be obtained both qualitatively and quantitatively. This study was analyzed using non-parametric test (Kruskal–Wallis test) to determine changes in enamel composition after bleaching, whether there is a change in enamel composition or not. Results: The results of this study showed a p-value of 0.406 (p < 0.05; significant). The result shows a change in the composition of dental enamel after bleaching using 35% H2O2. Conclusion: Application of 35% H2O2 as the bleaching agent does not have a significant influence on changes in the composition of the elements contained in the enamel.

An Analysis of Dental Enamel after Bleaching using 35% Hydrogen Peroxide with Energy-dispersive X-ray Spectroscopy