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Irradiation Tumors were irradiated with a single dose of 10 Gy at 7 days after implantation, when the diameter reached approximately 7-8mm. The mean tumor volume at irradiation was about 200mm3. Irradiation was performed with a Stabilipan2 X-ray machine (Siemens) operating at 180 kV and 20mA with a 0.5mm copper filter, giving a doserate of 1.80Gy/min. An adjustable cylindrical cone 20 mm in diameter was used to limit the radiation beam to the tumor. During irradiation, the mouse was restrained without anesthesia.

31P magnetic resonance spectroscopy for estimating the radiation response of a murine mammary tumor