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espanolEn diferentes regiones de Latinoamerica la infeccion por T. cruzi y Leishmania se superponen, por lo cual se reportan infecciones mixtas circulantes, debido a esto; deben realizarse pruebas diagnosticas especificas para evitar reacciones cruzadas entre estas dos patologias. Objetivo: Determinar patrones de fluorescencia que permitan la diferenciacion entre Leishmaniasis, enfermedad de Chagas e infeccion mixta empleando epimastigotes de T. cruzi. Metodologia: Se empleo la tecnica de Inmunofluorescencia Indirecta utilizando epimastigotes de T. cruzi (TcV autoctono) como antigeno figurado frente a un panel de muestras de suero codificados como A, B, C y D correspondientes a pacientes con infeccion por: Leishmaniasis (A), Infeccion mixta por Leishmania y Chagas(B), Enfermedad de Chagas (C) y sin ninguna de las dos infecciones (D). Resultados: En los cuatro paneles de muestras se observaron diferentes patrones de intensidad de fluorescencia a nivel de membrana y nucleo de los epimastigotes de T. cruzi (TcV autoctono). Conclusiones: La tecnica de Inmunofluorescencia (IFI) con antigenos de epimastigotes de T. cruzi a demostrado utilidad en la diferenciacion entre enfermedad de Chagas, Leishmaniasis y/o infecciones mixtas por ambos parasitos en aquellas zonas donde la coexistencia de ambas es habitual EnglishIn different regions of Latin America, infection by T. cruzi and Leishmania overlap, for which mixed circulating infections are reported, due to this; Specific diagnostic tests must be performed to avoid cross reactions between these two pathologies. Objective: To determine fluorescence patterns that allow the differentiation between Leishmaniasis, Chagas disease and mixed infection using T. cruzi epimastigotes. Methods: The Indirect Immunofluorescence technique was used using epimastigotes of T. cruzi (autochthonous TcV) asfigurative antigen against a panel of serum samples coded as A, B, C and D corresponding to patients with infection by: Leishmaniasis (A) , Mixed infection by Leishmania and Chagas (B), Chagas disease (C) and without either of the two infections (D). Results: In the four sample panels, different patterns of fluorescence intensity were observed at the membrane and nucleus level of the epimastigotes of T. cruzi (autochthonous TcV). Conclusions: The Immunofluorescence technique (IFI) with T. cruzi epimastigote antigens has proven useful in differentiating between Chagas disease, Leishmaniasis and / or mixed infections by both parasites in areas where the coexistence of both is common.

Patrones diferenciales entre Leishmaniasis y chagas, empleando epimastigotes de trypanosoma cruzi