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Imaging techniques are used for diagnosing dermatologic conditions from the conception of dermatology as a separate medical specialty. Nowadays, they can be combined with biochemical-molecular techniques to serve as even better diagnostic tool. Direct immunofluorescence (DIF) is a golden standard for routinely diagnosing autoimmune blistering diseases. DIF images can be visualized with short arc mercury lamp-operated microscopy, blue light-emitting diode technology-operated microscopy and laser scanning confocal microscopy. Advanced microscopy methods, such as ex vivo confocal laser scanning microscopy, atomic-force microscopy/scanning-force microscopy, multiphoton microscopy, super/high resolution techniques known under acronyms STED, GSDIM/dSTORM (Ground State Depletion followed by Individual Molecule return/direct Stochastic Optical Reconstruction Microscopy) can conceptually be applied to every-day laboratory practice. Three-tier approach to diagnosing individuals suffering from dermatologic ailments, namely clinical evaluation, imaging techniques, biochemical-molecular techniques is still indispensable in managing dermatologic patients.

frontiers in medical case reports

Imaging Techniques for Both Diagnosing Individuals with Dermatologic Conditions and Doing Clinical Research: A Selection of Our Contribution to this Field