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In the present investigation, Streptosporangium sp. (AI-21) a new soil borne isolate was characterized against food borne bacteria isolated from thirty-five food samples. Based on the morphological and biochemical characteristics, it was found that all food borne bacteria belong to Escherichia, Pseudomonas, Salmonella, Staphylococcus and Bacillus group of microorganisms. Colony morphology and biochemical characterization was the base of identification of actinomycete species. The actinomycete isolate was grown at a mass level by inoculum build up in mother flasks. Extraction of antibacterial substance from isolated actinomycetes was done by solventextraction method. Ethyl acetate extract exhibited activity against Staphylococcus sp. and Escherichia sp. The MICs of this extract was recorded in the range of 512 μg/ml to 2048 μg/mL. A maximum zone of 23.15mm was observed against Staphylococcus sp. The study not only targeted food borne isolates but also suggested how soil could be explored for the extraction of bioactive molecules by actinomycetes. This study could be a platform for new drug discovery.

In vitro antibacterial characterization of Streptosporangium sp. (AI-21) a new soil isolate against food borne bacteria