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This study aimed to assess the toxicity of ammonia on clam Cyclina sinensis and the post-exposure recovery. With increased exposure to TAN, the alkaline phosphatase (AKP) activities after exposure showed a trend of growing initially and subsequently decreasing, whereas the AKP activities after post-exposure recovery showed an increasing trend. The AKP activities after post-exposure recovery were significantly higher than those in control. The acid phosphatase (ACP) activities in T1 and T2 after post-exposure recovery were higher than those in the control, whereas the ACP activities in T3, T4, and T5 after post-exposure recovery were significantly higher than those in the control. The lysozyme (LZM) activities in T1 and T2 after exposure were significantly higher than those in control, whereas the LZM activities in T3, T4, and T5 after exposure were significantly lower than those in the control. Overall, ACP and LZM in the clams exposed to a low level of TAN (≤ 40 mg/L) can recover to the normal levels completely. However, a 48h recovery period scarcely seems adequate to compensate for AKP, ACP, and LZM activities in the clams exposed to a high level of TAN (&gt; 40 mg/L).

Effects of acute ammonia exposure and post-exposure recovery on nonspecific immunity in Clam Cyclina sinensis