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The unplanned application of pesticide in paddy crop field to enhance paddy production could cause adverse impact on the environment. The present study was aimed to isolate the bacteria prevalent in profenofos applied soil to determine their profenofos degrading ability and its assay of the degraded metabolites. The dominant bacteria isolated from paddy crop field soil were Bacillus cereus and Aneurinibacillus migulanus. These bacteria were spiked with profenofos. After 36 hours of incubation in soil the extract were analysed by GCMS. Profenofos degradation was enhanced by Aneurinibacillus migulanus (99.45 % of profenfos degraded) compared to Bacillus cereus (98.01 %) and control (88.46%). Thus Aneurinibacillus migulanus could be used as bioagents to degrade profenofos.