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RNA extraction technology is essential for molecular biology research. Targeting at microRNA or long non-coding RNA to diagnose or estimate the prognosis of various tumors are widely proceed. The fundamental step of such research is isolating high quality RNA of cells. Unfortunately, ribonucleases (RNases) that exist in almost all cells are responsible for the degradation of RNA, which results in concentrations of RNA not being able to meet experiment requirements, especially under the circumstance of raw material shortage. This means it is of great significance to isolate high concentrations of cells' or tissues' RNA. Also the traditional method of eliminating ethanol consumes too much time. Hence, a simple and efficient method was utilized during our research, which can not only produce total RNA of better quality, but also save RNA extraction time. One simple and efficient technical method of total RNA extraction