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Agarose gel electrophoresis is a common method for detecting the size of a nucleic acid sequences and judging whether they are smaller or larger than expected, whether there are other miscellaneous sequences, and whether the polymerase chain reaction (PCR) results are satisfactory. As the speeds of migration are different when nucleic acid sequences with different size are performed electrophoresis at the same voltage, the nucleic acid sequences are separated accordingly. Nucleic acid dyes will then be added to the agarose gel for the color of the image. Most nucleic acid dye, especially ethidium bromide (EB) has strong mutagenicity and toxicity, and EB is considered a hazardous waste and must be disposed of appropriately. Therefore one must be especially careful when handling agarose gels containing it. Although there are some alternatives to EB such as gel red, gel green, GoldViewna and the like, their safety is still relative, so an effective method to maintain less contact with the nucleic acid dye and to get clearer imaging is required. This study showed a new reagent loading method, which reduced direct contact with the dye, cut down the dosage, and can be widely used for nucleic acid gel electrophoresis.

The improved method in agarose gel electrophoresis of nucleic acid