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The role and importance of medicinal and aromatic plants is due to the antioxidant properties of its components, usually associated to a wide range of amphipathic molecules, broadly termed Phenolic compounds. Therefore, the research aims to increase the production of active medicinal compounds through the treatment of callus Gardenia jasminoides L plantlets with two different types of lighting and different concentrations of polyamine, and putrescine. The experiment was conducted in the tissue culture laboratory belonging to the department of plant production techniques, AL-Musaib technical college, the experiment included two factors, the first included two sources of Lighting type, the regular light (fluorescent) and LED light (18 red: 2 blue), and the second factor adding putrescine in three concentrations (0.5, 1, 1.5) mg.L in addition to the control concentration, Some phenolic substances (Coumaric, Ferulic, Caffeorylquanic, Sinapic acid, Tannic acid) were estimated in Gardenia jasminoides L callus using the high-performance liquid chromatography technique Hplc. Data was analyzed using Genstat statistical program, and the averages were compared according to the LSD test at 0.05. The results showed that the LED lighting treatment was significantly excelled in the concentration of all the measured compounds, and the putrescine treatment at a concentration of 0.5 mg.L gave the highest concentration of the compounds (Ferulic, Caffeory lquanic, Sinapic), while the concentration 1 mg.L gave the highest concentration of the compounds (Coumaric, Tannic acid). Also, the Bi-interaction (LED + Putrescine at concentration 0.5 mg.L) gave the highest concentration of compounds (Ferulic, Caffeory lquanic, Sinapic) in Gardenia jasminoides L callus.

Effect of Putrescine and the Lighting type on Gardenia jasminoides L. callus content of some active medical compounds