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stability study of Modified MustaTriphaladi Avaleha was done for 12 months (1 year) to know the stability of formulation in reference of its phyto-constituents and microbial growth therein. Modified MustaTriphaladi Avaleha have been used in Thalassemia children as an adjuvant therapy prepared with various herbal product. Methods: Modified Musta-Triphaladi Avaleha was made in Pharmacy with standard classical method (Avaleha Kalpana) as per AFI part-1 (The Ayurvedic formulary of India, part-1 partA) In present study, stability with respect to its Microbial profile of Modified Musta-Triphaladi Avaleha carried out. Avaleha was stored in 2 plastic containers during different climatic condition. Avaleha were studied at regular intervals for a period of 1 month to analysis Mycological findings and presence of Microorganisms by wet mount preparation and Gram stain test respectively. Results: At the end of study both Avaleha container has not present of microbes after 1 year of preparation, even in different climate and temperature. Conclusion: Since it is traditional Avaleha kalpana, the shelf life period was about one year (Sharangdhar Samhita) The presented study reflects that Microbiological findings of Modified Musta-Triphaladi Avaleha was negative at room temperature, warm and cold, dry and humid condition. INTERNATIONAL AYURVEDIC MEDICAL JOURNAL Bhumi Mori et al: Stability Study of Modified Musta-Triphaladiavaleha With Respect to Base-Line Microbial Profile Used in Thalassemia Major doi:10.46607/iamj.0208102020 | IAMJ October 2020 | www.iamj.in 4579

STABILITY STUDY OF MODIFIED MUSTA-TRIPHALADIAVALEHA WITH RESPECT TO BASELINE MICROBIAL PROFILE USED IN THALASSEMIA MAJOR