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A field experiment was conducted to evaluate the effect of Rhizobium inoculation methods and the effect of different levels of N fertilization on nodulation, nitrogen fixation and yield of Phaseolus vulgaris L. The N treatment produced a seed yield significantly higher than that of the other treatments. Inoculation treatments had no significant effect on nodule number and dry weight, even when compared to the noninoculated and N controls. This finding was attributed to a high number of Rhizobium phaseoli in the soil. The applications of N and inoculation did not significantly affect the N percentages of foliage and grain or nitrogenase activity. Serological identification of nodules indicated that the native strain UPRM 6000 farmed the largest percentage of nodules (41%), followed by R. phaseoli 127K44 (34%), 127K17 (13%) and 127K12b (10%). The serogroup distributions of inoculated and N fertilized treatments differed significantly from the noninoculated control. Inoculation methods (seed vs. soil) did not differ significantly in either nodulation or yield responses.

Inoculación de Phaseolus vulgaris con Rhizobium en Lajas, Puerto Rico