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Both extraction of phenolic acids eluted from barley straw in rivers/lakes and analytical method for the extracted phenolic acids using ultra high performance liquid chromatography (UHPLC)-mass spectroscopy (MS) were developed. Then, the analytical recovery, accuracy and precision of the developed analytical method were evaluated based on the quantitative/qualitative analysis. According to the chromatograms, seven phenolic acids eluted from barley straw were clearly separated in the order of Gallic acid > 4-Hydroxybenzoic acid > Vanillic acid > Caffeic acid > Syringic acid > Coumaric acid > Ferulic acid, and five phenolic acids except Gallic acid and Caffeic acid were found to have analysis recovery of 80.4~112.8%. Four phenolic acids (4-Hydroxybenzoic acid, Vanillic acid, Syringic acid, and Ferulic acid) presented in rivers/lakes displayed high linear correlation (R 2 ) values (i.e., 0.998~1.0) of calibration curves within the wide detection ranges, and had relatively low limit of detection (LOD) and limit of quantitation (LOQ). From the results of this study, the developed pretreatment method and analytical method were confirmed to effectively extract and analyze phenolic acid mixtures eluted from barley straw in complex environment matrix such as rivers/lakes.

Development of Extraction and Analytical Methods of Phenolic Compounds Degraded from Barley Straw in River