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: The feasibility of perchlorate reducing-bacteria isolated from the sludge of an anaerobic digester was determined using ammonium perchlorate in aqueous medium. Growth kinetics of the two perchlorate reducing bacteria including Rhodococcus sp. YSPW01 and YSPW02 were investigated using acetate as the electron donor in batch experiment. The growth of YSPW01 and YSPW02 reached a steady-state at 26 and 9 h, respectively. The initial perchlorate concentration was completely reduced within 8 and 7 h by YSPW01 and YSPW02, respectively. The reduction rates were 2.1 and 15 mg L -1 h -1 for YSPW01, and 3.2 and 15.5 mg L -1 h -1 for YSPW02, at 1:1 and 5:1 ratios of acetate:perchlorate (w:w), respectively. In this study, the bacteria Rhodococcus sp. YSPW01 and YSPW02 demonstrated a potential for the perchlorate reduction, which could be further investigated for develo-pment of an efficient strategy to treat the perchlorate contaminated waters. Key Words : Ammonium Perchlorate, Perchlorate Reducing Bacteria, Newly Isolated Bacteria, Rhodococcus sp., Batch Experiment

Reduction of Highly Concentrated Perchlorate in Aqueous Medium by Newly Isolated Bacterial Strains Rhodococcus sp. YSPW01 and YSPW02