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This study was performed to determine optimum conditions of semi-continuous cultivation of chlorella sp. FC-21 cultivated under red light emitting diode (LED). Semi-continuous cultivation was conducted using red LED because red LED was found to be the best light source for chlorella sp. FC-21. During cultivation, phosphate and nitrogen were quickly diminished where cell concentration of chlorella was inversely proportional to the concentrations of phosphate and nitrogen in culture solution. To increase the period of dilution of culture solution, additional amounts of phosphate and nitrogen were inserted in the culture solution to increase the concentrations of phosphate and nitrogen. The cell concentrations of chlorella increased in the modified culture, but cell diameter was diminished as the dilution of culture was periodically conducted. When considered the cell concentration and cell diameter during the cultivation, amount of biomass produced was maintained constant.

daehan hwan'gyeong gonghag hoeji/daehan hwan-gyeonggonghakoeji

Optimum Semi-Continuous Cultivation of Chlorella sp. FC-21 for Production of Biomass: Light Emitting Diodes as a Light Source and High Concentrations of Nitrogen and Phosphate in Culture Media