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Jeonbuk National University, Iksan Campus, Bio Food Technology Ma-dong, Iksan 570-752, KoreaABSTRACT: Lentinula edodes liquid spawn growth under explosive aeration (supplying air with tiny bubbles) and soybean mealaddition to liquid culture medium were investigated in terms of mycelial growth and residual free sugar content. The twotreatments were effective for homogeneous culturing of mycelial spawn and for separating colonies during multiplication after anexponential growth period without limiting sustaining nitrogen nutrients. The mycelial growth and carbon dioxide concentrationwere greatest on the 13th day since the inoculation. At 12th day, however, free sugars were almost depleted in the upper part ofthe liquid medium. Total nitrogen content within precipitated mycelia was the highest at the 13th day. Chitin and sucrose contentsin the mycelia were the highest at the 18th day, but ergosterol content became highest at 22 days. These results suggest thatLentinula edodes liquid spawn is ready in 18 days after inoculation.KEYWORDS :

Changes of Nutrients in Media and Mycelia on Liquid Spawn Culture of Lentinula edodes