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(Pleurotus ostreatus), (Pleurotus eryngii), (Flammulina velvtipes) (Hericium erinaceum), (Lyophyllum ulmarium), (Pleurotus cornucopiae), (Lentinus lepideus), (Agrocybe cylindracea)    (spent mushroom compost, SMC) ${\alpha}$ -amylase (EC 3.2.1.1), lignin peroxidase (EC 1.11.1.14), laccase (EC 1.10.3.2), xylanase (EC 3.2.1.8), ${\beta}$ -xylosidase (EC 3.2.1.37), ${\beta}$ -glucosidase (EC 3.2.1.21)  cellulase (EC 3.2.1.4)   buffer .  0.25% Triton X-100    xylanase  SMC 153 U/g    laccase  SMC 8.0 U/g  . ${\alpha}$ -amylase (3.6 U/g) cellulase (3.2 U/g)  SMC   .  SMC  laccase cellulase          . 【Recovery of ${\alpha}$ -amylase (EC 3.2.1.1), lignin peroxidase (EC 1.11.1.14), laccase (EC 1.10.3.2), xylanase (EC 3.2.1.8), ${\beta}$ -xylosidase (EC 3.2.1.37), ${\beta}$ -glucosidase (EC 3.2.1.21) and cellulase (EC 3.2.1.4) from spent mushroom composts (SMCs) of Pleurotus cornucopiae, Pleurotus ostreatus, Pleurotus eryngii, Hericium erinaceum, Lyophyllum ulmarium, Agrocybe cylindracea, Lentinus lepideus, and Flammulina velvtipes were investigated using different extraction buffers. The maximum recovery of the enzymes was mostly detected in SMC extracts with tap water and 0.25% Triton X-100 by shaking incubation (200 rpm) for 2 h at $4^{\circ}C$ . The xylanase (152 U/g) and laccase (8.1 U/g) activities were the highest in SMC extracts from F. velvtipes and P. eryngii. In addition, high enzymatic activities of ${\alpha}$ -amylase (3.6 U/g) and cellulase (3.4 U/g) was detected in SMC extract of A. cylindracea. Futhermore, cellulase and laccase activities of SMCE from P. eryngii were compared to commercial enzymes.】

Optimal Extraction and Characteristics of Lignocellulytic Enzymes from Various Spent Mushroom Composts