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Starting in the 1950s, two other separation processes that use open column chromatography started to be applied: size exclusion and bioaffinity. An important contribution to both these developments involved the commercialization of highly porous dextran and agarose gels, followed soon after by the preparation of porous organic polymers with similar properties. This chapter of “Pillars of Chromatography” describes the early days of both these separation techniques.

Separações em colunas abertas: cromatografia por exclusão e por bioafinidade