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Studies show that the association of diazotrophic bacteria with grasses can result in reduced need for nitrogen fertilization. However, it is necessary that the pesticides applied be selective to these microorganisms so that these rhizobia can express their whole potential of atmospheric nitrogen fixation. The objective of this study was to identify pesticides used in sugarcane crop that do not affect growth or cause harm to the ability of biological nitrogen fixation (BNF) of the diazotrophic bacterium Gluconacetobacter diazotrophicus. Five insecticides (imidacloprid, fipronil, thiamethoxam, endosulfan and carbofuran) were tested in their respective doses regarding the commercial impact on the growth of bacteria in liquid medium DIGs. To this end, the variables support capacity growth ("carrying capacity") in the culture medium used, duration of lag phase, and generation time were determined. The impact of pesticides on nitrogenase activity of G. diazotrophicus grown in semi-solid LGI-P-free N was also evaluated. None of the insecticides tested significantly affected the ability to growth support of G. diazotrophicus. However, increments in the duration of the lag phase observed in the presence of endosulfan and thiamethoxan, as well as in the generation time, with carbofuran, showed the occurrence of inhibitory effects of these insecticides on the growth of G. diazotrophicus under the conditions tested. None of the insecticides caused deleterious effect on BNF in vitro of G. diazotrophicus. Crescimento e fixacao biologica de nitrogenio de Gluconacetobacter diazotrophicus na presenca de inseticidas utilizados na cultura da cana-de-acucar

Crescimento e fixação biológica de nitrogênio de Gluconacetobacter diazotrophicus na presença de inseticidas utilizados na cultura da cana-de-açúcar