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The objectives of this work were to enumerate total coliforms and Escherichia coli, that is indicators of fecal contamination, comparing the achieved results with the current standards of national identity and quality, and also to perform the serological confirmation of isolated strains E. coli in chicken thigh, obtained in establishments with and without sanitary inspection, commercialized in the city of Rio de Janeiro. In the enumeration of the microorganisms, the miniaturized methodology was performed with culture media for rapid detection, based in fluorogenic and chromogenic substrates in their composition. Twelve of the 30 analyzed samples presented most probable number (MPN) of E. coli varying from <3 to 2.9x10 7 /g, with six (20%) considered improper for human consumption in accordance with the current legislation. From the 150 colonies suspected and confirmed with biochemical tests, 18 (12%) were tested positive for enteropathogenic E. coli (EPEC) and enteroinvasive E. coli (EIEC), taken from 15 different samples. From 17 colonies suspected for enterohaemorrhagic E. coli (EHEC), no strain belonged to this group. The results indicate that the existence of a service of efficient inspection, in all the stages of the technological process of the poultry, is very important to supply a product with quality and safety, without risking the health of the consumer.

Coliformes totais e Escherichia coli em coxas de frango comercializados no Rio de Janeiro