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Using the cyst fluid and the total antigens of Taenia crassiceps and positive and negative bovine control sera, some standardization parameters of one ELISA were compared, in order to evaluate its performance in the diagnosis of the bovine cysticercosis. The referred parameters were different antigen concentrations, dilutions of sera and conjugated, plate marks and blocking substances. The 1:25 dilution sera and 1:2.500 dilution conjugated for the cyst fluid antigen and 1:5.000 for the total antigen, the skimmed milk and the largest antigens concentrations (1, 2 and 4 µg/well) improved the differentiation among positive and negative sera, consequently, they were the best criteria for the bovine cysticercosis determination employing the ELISA.

Ensaios de padronização do teste ELISA para diagnóstico da cisticercose bovina utilizando antígenos de larva de Taenia crassiceps