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The aim of this research was to evaluate the interactivity of the combinations florfenicol (FFC) + thiamethoxan (TH) and enrofloxacine (ENRO) + toltrazuril (TOL) for two fish species, pacu (Piaractus mesopotamicus) and mato grosso (Hyphessobrycon eques), a snail (Pomacea canaliculata), a duckweed (Lemna minor) and a microcrustacean (Daphnia magna), which are bioindicators. For this, organisms were first acclimated in a bioassay room and then exposed to the abovementioned substances to verify their sensitivity and after that, in the definitive tests, they were exposed to nominal drug concentrations, in static system. Toxicity of each combination was evaluated using the lethal concentration of 50% (LC50) of each pure drug and the concentration of each drug responsible for LC50 of the combination, for each organism. The TH + FFC combination showed antagonistic toxicity for the pacu, the mato grosso, the duckweed and the snail but it was synergistic for the microcrustacean. The TOL + ENRO combination was synergistic for the pacu, the duckweed, the snail and the microcrustacean but it was antagonistic for the mato grosso fish. TOL + ENRO must be used with caution because this can synergistically interact and affect several bioindicators.

Avaliação ecotoxicológica de associações de fármacos utilizados na aquicultura