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espanolSe evaluo el efecto de celulas de granulosa preincubadas sobre la maduracion in vitro y pro- duccion de hormonas esteroides en vacas. Las celulas de granulosa de los ovarios fueron culti- vadas empleando el M-199 modificado con sales de Earle. Se anadio 1% de albumina de suero bovino y 5% de suero fetal de ternero. Tambien se incluyo un 0,8% de antibioticos. Los cultivos fue- ron incubados a 37°C con 95% de aire y 100% de humedad relativa. Se registraron diferencias sig- nificativas entre las concentraciones de progesterona y estradiol obtenidas con los niveles de inclsuion de FSH y LH. Puede concluirse que es necesario suplementar con hormonas el medio de cultivo para IVM en bovinos. EnglishThis study aimed to evaluate the effects of preincubated granulosa cells on in vitro maturation and steroid hormones production in cows. Granulosa cells from the ovaries were cultured using the modified M-199 with Earle's salts. 1% bovine serum albumin was added and 5% fetal calf serum. Also 0.8% of antibiotics was also included. Cultures were incubated at 37°C in a 95% air and 100% relative humidity. There were significant differences among values of proges- terone and estradiol concentration obtained for levels of inclusion of FSH and LH. It can be concluded that there is necessity for supplemen- tation of hormones in culture medium for bovine IVM.

In vitro maturation of bovine granulosa cells for steroid hormone production