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Materials and Methods Antimicrobial activity of 1% and 2% alexidine, 0.2% and 2% chlorhexidine, 0.2% cetrimide and 0.2%, 0.5%, was assayed on 1-week S. mutans biofilm formed on standardized coronal dentin blocks. Results of S. mutans biofilm antimicrobial activity by different protocols were, respectively, expressed as the kill percentage of biofilm and the term `̀ eradication’’ was used to denote the kill of 100% of the bacterial population. To compare the efficacies of the different protocols the Student t test was used, previously subjecting data to the Anscombe transformation.

Minimally invasive aesthetics through interdisciplinary orthodontic-restorative treatment