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Purpose: To investigate the combined effect of Carica papaya extract and mineral elements wound healing. Methods: Phosphate-buffered saline (PBS) and water extracts of unripe Carica papaya. papaya were analyzed for mineral element composition (Ca, calcium; Mg, magnesium; Na, sodium; Fe, iron; Mn, manganese; Zn, zinc; Ag, silver; and Se, selenium) using a microwave-assisted digestion procedure. Atomic absorption spectrophotometry was used for the analysis of the elements. Phytochemical analysis was conducted using standard protocols. Mice weighing 25 - 30 g were anesthetized, shaved and inflicted with wound at the dorsal region using a biopsy punch. The mice were treated topically twice daily with or without 5 mg/ml PBS or water extracts containing 100 or 200 µM Zn2+ or 1.0 or 0.5 µg/g Se2+. The standard (positive control) group was treated with solcoseryl and the negative control with deionised H2O. Wound area was monitored with a camera and evaluated by software. Results: PBS extract formulated with Se2+. (0.5 or 1.0 μg/g) took approximately 9 days for complete wound healing while negative control took 14 days and other treatment groups least 10 days. PBS extract contained 9 % protein but no tannin while water extract contained 0.58 % protein and tannins. Calcium (137.98 mg/100 g extract) constituted the most abundant macro-element while Se (32.14 mg/100 g extract) was the most abundant microelement in C. papaya pulp. Conclusion: Addition of selenium to C. papaya extracts augments the wound healing activity of the extracts.

Addition of Selenium to <i>Carica papaya</i> Linn Pulp Extract Enhances Dermal Wound Healing Activity