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Botanical larvicides have featured prominently as alternative to synthetic chemical insecticides which are less degradable and toxic to non-target organisms. The larvicidal potentials of the seed oils of Pterocarpus santalinoides and Tropical Manihot species (TMS 30572) were investigated in the laboratory against larvae of Aedes aegypti. The seed oil of each plant was extracted using n-hexane and was graded into different concentrations; 30, 60, 90, 120 and 150 ppm. The toxicity of each of the concentrations was evaluated against 3rd instar larvae of A. aegypti and tadpoles (Buffo spp) as non target aquatic fauna. Both oils were toxic to the larvae though at higher concentrations (120 ppm and 150 ppm) after 24 hours of exposure. The oil of P. santalinoides was more toxic to the larvae (LC50 104.0 ppm and LC90 184.5 ppm) than oil of TMS (LC50 113.5 and LC90 201.2) but the difference in the lethal doses was not statistically significant (P &gt; 0.05). However, mortality was not recorded at any of the graded concentrations in both oils against tadpoles. The results therefore suggest that the seed oils of both plants could be incorporated as botanical insecticides against mosquito vectors with high safety to non-target organisms.

Larvicidal efficacy of seed oils of Pterocarpus santalinoides and Tropical Manihot species against Aedes aegypti and effects on aquatic fauna