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L\'effet du stress hydrique osmotique induit par le polyethylene glycol (PEG) a ete etudie sur la germination et la croissance in vitro chez le cotonnier. Cinq concentrations differentes de PEG ont ete testees et les resultats ont montre que le PEG agit negativement sur la germination des graines. La longueur des racines et des tiges diminue avec les concentrations elevees de PEG dans le milieu de culture. La croissance des feuilles est inhibee a partir de 15 g/l de PEG dans le milieu de culture. Les parties aeriennes ont ete plus affectees par l\'effet du PEG que les parties racinaires. Le fletrissement des plantules est observe a partir de 35 g/l de PEG dans le milieu de culture. The effect of water stress induced by polyethylene glycol (PEG) has been studied on germination and seedling growth of cotton. Five different concentrations of PEG have been tested and the results showed that PEG concentrations negatively influence seeds germination. The length of roots and stems decreases with high concentrations of PEG in medium culture. The growth of leaves is inhibited from 15 g/L of PEG in medium culture. Aerial parts have beenmore affected by water stress than the roots. Plantlet whithering is observed from 35 g/L of PEG in medium culture. Keywords : cotton, culture in vitro, PEG, water deficit Sciences & Nature Vol. 5 (1) 2008: pp. 81-87

sciences and nature. série a, biosciences agronomie environnement biotechnologie

Réponse physiologique au stade juvénile du génotype R405-2000 de cotonnier (Gossypium hirsutum L.) au déficit hydrique induit par le polyéthylène glycol