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This study reported production of lipase by Bacillus thuringiensis and Lysinibacillus sphaericus . Bacteria isolates were screened on Bushnell-Hass Mineral Salt medium containing 1% PMS for oil degradation. Two potent isolates were identified using 16S rRNA as Bacillus thuringiensis and Lysinibacillus sphaericus . They were cultured for lipase production in a submerged medium. The crude lipases extracted were used for degradation of PMS. Optimum degradation of PMS 44.5% and 37.4% were obtained by lipase from Bacillus thuringiensis and Lysinibacillus sphaericus respectively at pH 7 and 35 in 20 days. This study therefore presented the use of bacterial lipase in degradation of PMS as a simple and effective approach. Keywords : Bacillus spp., biodegradation, lipase,

Biodegradation of premium motor spirit (PMS) by lipase from Bacillus thuringiensis and Lysinibacillus sphaericus